Logo_IRSEA IRSEA
Research Institute in Semiochemistry and Applied Ethology Language

The Team

Laurent BOUGRAT Head of the Department

Laurent BOUGRAT

M. Sc. in Chemistry
Cécile FROSINI Biotechnology Manager

Cécile FROSINI

PhD in Neuroscience
M. Sc. in Biotechnology

Camille CHABAUD Technician

Camille CHABAUD

B. Sc. in Experimental
and Analytical Biology




Contact us


E-mail


Phone

+33 490 755 704






Presentation of the department




The department is divided into two units, a chemistry and a biotechnology unit.


- The chemistry unit is subdivided into two fields of activity, analytical chemistry and galenics. Analytical chemistry aims at indentifying pheromone messages and galenics aims at making these messages readily available.
- The biotechnology unit is subdivided into two main activities : molecular biology and biochemistry. Molecular biology studies and produces, by recombinant technology, the proteins involved in chemical communication. Biochemistry is in charge of the characterization of these proteins for their immunological and functional properties.






Operating fields




The work of the chemistry laboratory is divided into two lines of research :

- The identification of pheromones (analytical chemistry). The most appropriate medium to capture the pheromonal message is selected, in relation with the relevant department. The sample is then analysed with gas chromatography combined with mass spectrometry (GC/MS) to obtain its structure. Adsorption, headspace and thermo-desorption techniques are regularly used for the processing of all samples to be analysed.

- The development of systems permitting the pheromone to be readily available according to the obligations the environment imposes (Galenics). Techniques of active diffusion such as electrical diffusers and passive diffusion such as polymers and gels have been developed in our laboratory.



The IRSEA's biotechnology laboratory's function is to study and analyse the proteins involved in animal chemical communication. The IRSEA is now a specialist in this field and has, for a long time, had an interest in studying pheromones; the molecules of communication. Pheromones are composed of several chemical components acting as a code in the animals' olfactory system. These chemical signals are processed by different types of proteins : olfactory receptors or vomeronasals and the olfactory proteins linking the odors or pheromones (Odorant Binding Protein and Pheromone Binding Protein). These proteins are essential in a proper integration of the communication chemical signals by the animal.

Moreover, other proteins that seem to have an effect on behaviour, such as MUP (Major Urinary Protein) and Fel d 1, are under study .

Studying these proteins is therefore crucial in achieving a better understanding of animal communication and its influence on their behaviour. This makes the ellaboration of new or more efficient products, to improve animals' well-being and behaviour control, possible.






Chemistry unit





Ø ANALYTICAL MEANS








GC/MS with headspace
GC/MS with Thermal Desorption System

HSGC
THGC






Examples of chromatograms obtained
Results of library research

GC MS
Results of a research in GC MS library


Ø GALENIC


  • Developed and marketed forms

Galenic shapes
Galenic forms
Equanimity Avizen
Equanimity Nasal Gel Gel diffuser block



  • Products in progress


Diffusing block
Towelette

Diffusing block
Towelette
Scratching post





Biotechnology Unit




Ø    ACTIVITIES


  • Biochemistry
  • Immunology
  • Molecular biology
  • Cell culture


Ø     TECHNOLOGIES


  • Electrophoresis (SDS-Page, Native-Page et IsoElectroFocalisation)





Electrophoresis




  • Western-Blots and Lectin-Blots





  • Mass spectrometry MALDI-TOF (total mass, mass peptidic card, glycan analysis)


Maldi Toff Mass Spectrometry


  • Linking tests on native gel and in spectrofluorimetry


  • Immuno-assays (ELISA, EIA)


ELISA test

  • Immunopurification
  • Molecular biology techniques : transcriptome analysis (PCR and RT-PCR), pathogen detection.


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