The department is divided into two units, a chemistry and a biotechnology unit.
- The chemistry unit is subdivided into two fields of activity, analytical chemistry and galenics.
Analytical chemistry aims at indentifying pheromone messages and galenics aims at making these messages readily available.
- The biotechnology unit is subdivided into two main activities : molecular biology and biochemistry.
Molecular biology studies and produces, by recombinant technology, the proteins involved in chemical communication.
Biochemistry is in charge of the characterization of these proteins for their immunological and functional properties.
Operating fields
The work of the chemistry laboratory is divided into two lines of research :
- The identification of pheromones (analytical chemistry).
The most appropriate medium to capture the pheromonal message is selected, in relation with the relevant department.
The sample is then analysed with gas chromatography combined with mass spectrometry (GC/MS) to obtain its structure.
Adsorption, headspace and thermo-desorption techniques are regularly used for the processing of all samples to be analysed.
- The development of systems permitting the pheromone to be readily available according to the obligations the environment imposes (Galenics).
Techniques of active diffusion such as electrical diffusers and passive diffusion such as polymers and gels have been developed in our laboratory.
The IRSEA's biotechnology laboratory's function is to study and analyse the proteins involved in animal chemical communication.
The IRSEA is now a specialist in this field and has, for a long time, had an interest in studying pheromones; the molecules of communication.
Pheromones are composed of several chemical components acting as a code in the animals' olfactory system.
These chemical signals are processed by different types of proteins : olfactory receptors or vomeronasals and the olfactory proteins
linking the odors or pheromones (Odorant Binding Protein and Pheromone Binding Protein).
These proteins are essential in a proper integration of the communication chemical signals by the animal.
Moreover, other proteins that seem to have an effect on behaviour,
such as MUP (Major Urinary Protein) and Fel d 1, are under study .
Studying these proteins is therefore crucial in achieving a better understanding of animal communication and its influence on their behaviour.
This makes the ellaboration of new or more efficient products, to improve animals' well-being and behaviour control, possible.
Chemistry unit
Ø ANALYTICAL MEANS
GC/MS with headspace
GC/MS with Thermal Desorption System
Examples of chromatograms obtained
Results of library research
Ø GALENIC
Developed and marketed forms
Galenic forms
Equanimity Nasal Gel
Gel diffuser block
Products in progress
Diffusing block
Towelette
Scratching post
Biotechnology Unit
Ø ACTIVITIES
Biochemistry
Immunology
Molecular biology
Cell culture
Ø TECHNOLOGIES
Electrophoresis (SDS-Page, Native-Page et IsoElectroFocalisation)
Western-Blots and Lectin-Blots
Mass spectrometry MALDI-TOF (total mass, mass peptidic card, glycan analysis)
Linking tests on native gel and in spectrofluorimetry